Speakers - AFHWC2025

Abstract

This study evaluated the agronomic characteristics and phytochemical composition of two double haploid barley lines (DH1, DH2) and their respective parents (P1 = Ardhaoui, P2 = Roho), under conditions of optimum moisture (100% field capacity) and water stress (50% field capacity), using a high-throughput imaging platform. Growth traits were sig-nificantly reduced under the applied water stress. The DH lines showed greater plant length (40.25 mm, 43.75 mm for DH1, DH2) and higher seed yield (NSS = 47.25, 48.25) compared with the parents. DH1 had the highest number of fertile tillers (NFT = 7). Under stress, proline (1.4 g kg-¹ FW in DH1) and sugars (4.1 g kg-¹ FW in DH2) increased, while DH lines showed high potassium concentrations as well as increased chlorophyll and protein levels under both treatments. This suggests that DH lines might efficiently cope with water stress. Non-destructive 3D High Throughput Phenotyping confirmed the su-perior performance and drought tolerance in DH lines. The superior performance of DH1 and DH2 under water stress conditions underscores their suitability for cultivation in drought-prone environments and their potential use in future breeding programs target-ing climate resilience.